The move, which the former president signed off on one week before leaving office last month, blinded Colorado officials and raised questions about political retaliation, the Associated Press reported.
Mr. Trump announced at a 2020 rally in Colorado Springs that the U.S. space command would remain at the Peterson Air Force base in the city.
But after Republican Senator Cory Gardner of Colorado lost his seat, Trump decided to move headquarters to the U.S. Army’s Redstone Arsenal in Huntsville, Alabama.
Mr Trump won 62 percent of the vote in Alabam in the 2020 presidential election, and the state also elected a pro-Trump senator, Tommy Tuberville.
Now, the Department of Defense’s inspector general has announced an investigation into the resettlement to see if it complies with Air Force and Pentagon policies.
“It is imperative that we thoroughly review what I believe will be a fundamentally flawed process that focuses on bean counting rather than American domination of space,” Republican Congressman Doug Lamborn said.
Colorado’s two Democratic senators also welcomed the review.
“Moving space command will disrupt the mission while endangering national security and economic viability,” Michael Bennet and John Hickenlooper said in a joint statement.
US Space Command differs from the US Space Force, the sixth and newest branch of the US military established in 2019.
Space Command is not an individual military service, but a central command for all military space operations.
It was dissolved in 2002 and also revived in 2019.
The air force accepted bids from six final locations, including Huntsville, before Mr. Trump hinted that it would stay in Colorado.
Associated Press contributed to this report